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Thus, values for H3K4me3, H3K56ac, H3K36me3, H2Bub and H3K9me3 increase gradually with gene expression, at least up to mid expression levels, whereas values for H3K27me1, H3K27me2 and H3K27me3 show an opposite trend.
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When looking deeper into this issue, we found that this happened just in the subsets of low-mid expression genes.
The MVA plots show clearly that the variability around agreement is highest in the mid-expression levels, above the floor and below the ceiling, and provide a feel for the amount of scatter in the points that correspond to the respective standard deviations.
Our results show that change in fluorescence lifetime of the bound contrast agent can be used to assess the high to mid-level expression of HER2 expressing tumors in vivo in only one measurement.
Using HER2-specific Affibody conjugated with the Dylight750 fluorescent probe as contrast agent, we demonstrated in live animals that change in fluorescence lifetime of the bound contrast agent can be used to assess the high to mid-level expression of HER2 expressing tumors in-vivo in only one measurement.
Mid stage expression.
The mid-parent expression level of 2511 differentially expressed genes was significantly (p < 0.01) correlated with hybrid performance (PY) or heterosis (HY) for grain yield.
This difference was qualitatively assessed by dividing the genes into quartiles of equal number, ordered by observed values in AS, with the first quartile representing the highest expressing genes, the second quartile representing mid-level expression genes, and the third and fourth quartile defining the genes with the lowest levels of transcripts (Figure 1B).
The remainder of the differentially expressed genes showed a continuum of additive, mid-parental expression pattern among the hybrid-inbred triplets (see Additional file 10).
Genes that were more highly expressed in the paternal parent tended to exhibit mid-parent expression patterns in the hybrids [ 15].
In a former study we identified 1,999 genes differentially expressed between 21 parental inbred lines, which additive, mid-parental expression levels in seven-day-old seedlings were correlated (p ≤ 0.01) with MPH for GY field data of 98 hybrids [ 11].
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