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The addition of butyrate triggered solvent production in R1 as early as the mid exponential phase.
DM25 flask cultures were grown to mid exponential phase (OD420 0.03–0.04) after preconditioning strains as described for growth curves.
We studied the different expression profiles at early exponential phase (7 hours) and mid exponential phase (14 hours).
All strains were routinely cultured at 37°C in TSB broth (Oxoid, Hampshire, UK) until reaching mid exponential phase.
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The overnight cultures were diluted to OD600 0.1 and incubated until mid-exponential phase was reached.
Each transformant was grown to mid-exponential phase in MD media before harvesting and RNA extraction.
These cultures, harvested at mid-exponential phase, were used as inocula for U VI) reduction experiments.
An appropriate OD600 value (~0.6) for mid-exponential phase was determined using the previously defined growth profile (Fig. 3).
For Gram-positives, each strain was grown to mid-exponential phase then centrifuged at 3000g to collect cell pellets.
The S. mutans cultures were grown to mid-exponential phase (KU70), followed by RNA extraction.
Sporidia culture at mid-exponential phase was pelleted by centrifugation and thoroughly washed with water.
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