The two main MID clusters with non-biological process GO enriched terms were clusters 5 and 7. Cluster 5 was over-enriched with 57% of terms that included the term "regulation", and cluster 7 displayed under-enrichment in cell-cycle genes, phosphorylation, and regulation of cell proliferation and developmental processes.
The mid development cluster (MD) contained 168 genes (169 features) with expression peaking at 60 and 87 DAA and low expression at other stages of development.
One integral plasma membrane protein homologue and one expansin homologue showed similar patterns of expression in both apple and tomato and were selected in the mid development cluster in the apple microarray.
Progression in the accumulation of sugars and pigments in pink, green soft, and green hard berries of the mid-véraison cluster were followed to post-mid-véraison stage and the times the under-ripe berries reach sugar and color levels in red berries at mid-véraison (indicated by boxed text) were calculated [ 30 ].
The leaders of the mid-80s cluster of bands dubbed the Paisley Underground were Rain Parade, featuring the Roback brothers (one of whom would later form Mazzy Star).
In the mid-véraison cluster, low-SB berries were in pink to red stages with sugar levels at 14%, while high-SB berries were still at 8%.
In a mid-véraison cluster (50% of berries have changed color), uneven ripening among the individual berries indicates that the berries do not enter the ripening phase at the same time.
In contrast, cellular transport and transport mechanism functions are more highly represented in the mid-development cluster (2.6% vs 1.6 to 1.8%) at the time when fruit are taking up nutrients and water most rapidly.
Further, SB showed a good correlation with sugar levels of the berries in the mid-véraison cluster, indicating that berries with low seed content begin to accumulate sugars, the main criterion for the ripening onset, earlier than those with high-SB content.
To examine the composition of green, pink, and red berries in the mid-véraison cluster originating from berries with low and high seed content, we segregated the berries on the basis of their "seed weight-to-berry weight ratio (SB)", and refer to these as the low and high-SB groups hereafter.
The representation is as in Fig. 1 a. (B and C ) Tip60 enrichment around enhancer mid points of cluster 1 (B ) and cluster 2 (C ).
