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White boxes indicate dorsal, mid, and ventral pons populations of PAX3-expressing cells.
Distinct clusters of cells immunoreactive for PAX3 were found in the dorsal, mid, and ventral pons.
Cre expression under rat insulin 2 promoter (RIP2) has been found in mid and ventral brain and spinal cord.
For each marker, the same threshold was applied to each image across the dorsal, mid, and ventral pons regions for each section.
SG-induced apoptosis was also present in the OE of the mid and ventral septum lining the middle medial meatus in the distal nasal passages (T3 and T4).
However, this promoter also drives Cre expression in the mid and ventral brain (Wicksteed et al., 2010); and in subset of neurons in the spinal cord (Sun et al., 2010).
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High-powered (20x) pictures of the PAX3-expressing cells in each brainstem region (midbrain, dorsal pons, mid pons, and ventral pons) were taken, 1 3 images per region per section, until at least 6 sections were represented for each region.
Late fourth larval stage (L4) animals were identified based on age (42 hours after feeding L1 arrested animals), size, and a mid body ventral clearance with a dark shadow in its center, which correspond to the developing reproductive structures.
Middle femur with ventral surface has a comb of short thickened spines, ventral surface of mid and hind tibia of male without long hairs.
Figure 4 shows the average spectral sensitivity for: top dorsal and ventral hemispheric photoreceptors; and bottom mid-band rows 5 and 6 photoreceptors.
Table 1 shows the results for R1 cells measured in the dorsal and ventral hemispheres, and row 5 from the mid-band, Figures 4C G.
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