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We designed ten forward and ten reverse fusion primers using the first ten MID's from the Standard 454 Set.
That impressive growth will be paralleled by the penetration of several wireless technologies that are integral to the MID's functions: Wi-Fi, Bluetooth, and eventually ultra-wideband (UWB).
The change in HRQOL did not reach the established MID's representing a clinically significant change.
Hence a small number of MID's were used to uniquely barcode a large number of individuals [ 28].
The MID's are the sequence tags that allow individual identification; when both forward and reverse primers are tagged, individual identification can easily be achieved by selecting different combinations of MID's for each sample.
These MID's have been engineered by Roche to avoid misassignment of reads and they are tolerant to several errors [ 46].
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That all changed in the mid 90's of course.
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