To test how kinetochore-fiber dynamics modulate spindle shape, we generated long spindles without disrupting kinetochore-fibers by depleting the microtubule depolymerase Kif18a.
Changes in microtubule-associated proteins, such as microtubule-associated protein-4 and tau, can also affect the microtubule dynamics and modulate sensitivity to taxanes and vincas [ 46, 47].
These new and previously published results of experiments in which microtubule dynamics was directly modulated with inhibitors are at odds with the speculations that microtubule dynamics as such may be involved in T cell polarization, which speculations find support only in T-cell signaling studies [4], [5].
BtpA has also been found to modulate microtubule dynamics.
In cells, some microtubule-associated proteins (MAPs) and molecular motors can further modulate microtubule dynamics.
Because α-tubulin acetylation increases following microtubule assembly, it is suggested that PP1 facilitates the recruitment of HDAC6 to modulate microtubule dynamics.
Besides its important role in interference with TLR signalling BtpA has been shown to modulate microtubule dynamics and protect microtubules from drug-induced depolymerisation [ 30].
However, it is not known if disruption of EB protein interaction would modulate microtubule dynamics during mitosis as it does during interphase (reviewed in Tamura and Draviam, 2012).
Nevertheless, the endogenous and over-expression results suggest that mtr1p is not a +TIP protein, but does somehow modulate microtubule dynamics.
At the microtubule lattice, neuronal MAPs such as Tau and MAP2 can modulate microtubule dynamics (Matenia and Mandelkow, 2009), although their major roles may be to fortify the microtubule lattice stiffness (Felgner et al., 1997).
