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We next established a procedure for the automated, fluorescence microscopy-based identification of cells arrested in mitosis upon short-term (8 h) exposure to different mitotic inhibitors and apoptosis inducers.
The accuracy of this estimate will depend on the accuracy of microscopy-based identification of all Plasmodium species, the notification rate of malaria cases, and the proportion of persons with malaria who seek care at a health clinic.
Technical developments that will allow a more direct comparison with our data include protocols that optimize the time of tissue processing and sampling strategies, such as automated ultrastructural reconstructions, improved ultrastructural stereological quantifications and light microscopy-based identification of putative synapses (Denk and Horstmann 2004; Vanhecke et al. 2007) (see below).
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Carvalho, F. A. et al. Atomic force microscopy-based molecular recognition of a fibrinogen receptor on human erythrocytes.
Identification of novel pro-migratory, cancer-associated genes using quantitative, microscopy-based screening.
Gonzalez, I., Mateos-Langerak, J., Thomas, A., Cheutin, T. & Cavalli, G. Identification of regulators of the three-dimensional polycomb organization by a microscopy-based genome-wide RNAi screen.
Microscopy-based screens of the GFP-labeled protein collection (Huh et al. 2003) have also led to the identification of numerous novel LD-associated proteins (Natter et al. 2005; see below).
However, the identification of new proteins involved in its structural organisation is a more challenging task, because it requires microscopy-based assays that are much more laborious.
Harris, A. R., & Charras, G. T. Experimental validation of atomic force microscopy-based cell elasticity measurements.
Hua, Y., Laserstein, P. & Helmstaedter, M. Large-volume en-bloc staining for electron microscopy-based connectomics.
Information was sought on their perception of symptoms-based, RDT-based, and microscopy-based malaria diagnoses.
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