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Here, light sheet based microscopy would be an ideal solution for single molecule experiments in thick specimen [19], [20].
Replacement of light microscopy with fluorescence microscopy would be one of the immediate options for improving TB case detection in high-burden settings.
Ossicles created with SPION-labeled cells show several Prussian blue positive adipocytes (see Figure 5C), strongly suggesting their origin from human BMSCs, although electron microscopy would be needed to be definitive.
The ischemia caused by Raynaud's phenomenon, especially coupled with malformations caused by high levels of VEGF could cause capillary collapse and could be misinterpreted as loss of capillaries since nail fold microscopy would be unable to identify unperfused capillaries.
Thus a negative malaria result diagnosed by microscopy would be more reliable than a positive result.
To assess bacterial abundances on sclerotia, FISH (fluorescence in situ hybridization) in combination with confocal laser scanning microscopy would be a useful method (Grube and Berg, 2009).
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Therefore, additional methodological approaches such as fluorescence lifetime imaging microscopy (FLIM) would be required.
Here is where a subsequent fluorescence activated cell sorting (FACS) and/or complementary (e.g., microscopy) analysis would be useful.
Therefore for such connected or thick specimens the off-axis reflection mode of our lensfree microscopy platform would be a better choice.
In addition, through the use of laser capture microscopy it would be possible to determine the actual level of mtDNA damage within focal areas found in different layers of the skin.
Superresolution microscopy studies would be able to reveal detail at the nanometre scale and help elucidate the specific locations of mDia1 and mDia2, as well as other proteins associated with filopodial protrusion, within the structures with much greater accuracy.
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