For this purpose we combined intravital microscopy with the hypoxic mouse model.
After incubation in the dark at room temperature for 20 min, cells were observed via fluorescence microscopy with the appropriate filters.
Thin Te flakes show up with a progression of colors in reflected light microscopy with the thinnest crystals appearing as darker greens and blues on this silicon substrate.
The cells containing the MT-YFP/GLuc were positive on fluorescence microscopy, with the fluorescent signal confined to the cell membrane.
The structure and morphology of MIP-CMSs were characterized using field emission scanning electron microscopy with the energy dispersive spectrometer, transmission electron microscopy, and Fourier transformation infrared spectroscopy.
This allows us to correlate the GFP fluorescence signal detected in vivo by microscopy with the synthesis, turnover and assembly of COX.
Therefore, in studies of dynamic cellular processes, it would be ideal to be able to combine the capability of in vivo fluorescence video microscopy with the EM.
Calcium responses in P-NAP-treated oligodendrocytes were determined by confocal microscopy with the calcium indicator fluo-3 AM, and compared with control cultures.
Thereafter, they were incubated for 45 min with a 25 μM carboxy-H2DCFDA working solution (in DMSO PBS at 1 400 v/v) and immediately observed via fluorescence microscopy with the appropriate filters.
A technique for virtual fluid-stage high-angle annular dark-field scanning transmission electron microscopy with the multislice method is presented that enables the virtual imaging of model fluid-stage systems composed of millions of atoms.
A solution concept to some of these issues on the basis of current technology and already demonstrated concepts is presented and future possibilities in in situ and multi-dimensional microscopy with the new concept are laid out.
