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The activity of TRPV1 was measured both by a conventional fluorescence method to measure intracellular Ca2+ and digital holographic (DH) quantitative phase microscopy, which detects cell surface topology perturbations that follow to Ca2+ entry.
GFP-fusion-protein expression was monitored using fluorescence microscopy, which detects fluorescence of the GFP protein (Fig. 1a).
The confocal microscopy which detects structural changes of fibrin may be influenced by many factors including CAPE concentration, buffers, dyes, and WB clot size used for confocal microscopy.
MPM is a nonlinear microscopy which detects two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) in tissues when excited by a femtosecond laser.
Comparable results were obtained in cryosections of spleens stained with Giemsa and visualized under polarized light microscopy, which detects parasites because of the refringent properties of haemozoin (Fig. S2).
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In order to do so, we have used Confocal Laser Scanning Microscopy (CLSM), which detects the natural fluorescence of photosynthetic pigments, and we have determined the total and differentiated biomass of the cyanobacteria by means of an image analysis program, Image J v1.37 (CLSM-IA) [16].
To examine whether SagE protein was produced in the vaccinated fish, immunocolloidal gold electron microscopy was carried out, which detected SagE protein in the muscle tissues of pSagE-vaccinated fish but not in pCN3- or PBS-vaccinated fish.
This was verified by electron microscopy, which also detected more subtle differences between them: DCM cells contained abundant nuclear pore dilatation, while aWS and HGPS displayed frequent thickening of the nuclear membrane.
We further confirmed the piling of the cells in the PPL-transfectant cultures by transmission electron microscopy (TEM; Fig. 2F), which detected stratified cells in the PPL-transfected cultures but not in the mock-transfected cultures.
In low resource settings, even culture may not be available and diagnosis must be based on smear microscopy, which fails to detect nearly half of patients with TB (14 ).
With Q-banding, chromosomes are stained with quinacrine mustard or a related compound and examined by fluorescence microscopy, which is useful for detecting heteromorphisms (variants in chromosomal structural).
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