Samples were viewed by light microscopy to determine the presence of multilocular droplets.
After 48 h, cells harboring an expressing integrant were viewed by fluorescence microscopy based on GFP.
For the sampling on day 28, the mucus of the integument and biopsy of the gill arches were carried out separately, which were viewed by light microscopy used for research of ectoparasites.
The cells were viewed by epifluorescence microscopy (Olympus BX51, Japan).
The cells were viewed by fluorescence microscopy at 630× total magnification on a DM 4000B fluorescence microscope (Leica, Germany).
Basilar papillae immunohistochemically labeled for BrdU were viewed using brightfield microscopy and were imaged using a digital camera attached to a Zeiss Stemi SV 11 stereoscope.
Cells were rapidly washed three times with PBS and were viewed by fluorescent microscopy in order to carry out image analysis [17].
Stained cells were viewed by fluorescent microscopy (Olympus BX41) equipped with a 100x oil immersion objective, using excitation wavelengths of 530-550l (foRRXRX) and 360-370l (for DAPI).
After washing twice for 15 min, cells were viewed under fluorescent microscopy and also examined by flow cytometry after passing them through a cell-strainer (Falcon® 8235).
The slides were viewed by light microscopy using an Olympus BX50 microscope (Olympus Instruments, Melville, NY, USA) equipped with a digital camera Nikon DMX 1200 (Nikon, Melville, NY, USA).
