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Specimens for electron microscopy were prepared by deposition of GO suspensions on a holey carbon support film on a copper grid (a droplet of 50 μg/mL).
Specimens for transmission electron microscopy were prepared from the deformed micro-cantilevers using a dual-beam FIB.
Small bubbles of nitrogen, methane, or argon with an average radius of 50 nm, as measured by scanning electron microscopy, were prepared under atmospheric conditions.
Nanoparticle specimens, for inspection by transmission electron microscopy, were prepared by slow evaporation of one drop of a dilute aqueous solution of the particles on a carbon-coated copper mesh grid.
Samples for microscopy were prepared by pouring a drop of diluted dispersion fluid (0.001%w/w) on a quartz crystal platelet that was thereafter left to dry in an oven, or by putting a sample directly on the sampling area (5 × 5 mm) on a quartz crystal platelet.
Specimens for confocal fluorescence microscopy were prepared by pipetting 50 μL of the red fluorescently labeled microsphere suspension (FluoSpheres® NeutrAvidin® labeled microspheres; 0.04 μm; red fluorescent, excitation 580 nm, emission 605 nm; Molecular Probes, Invitrogen, Carlsbad, CA) onto the glass bottom of 35 mm glass bottom dishes (MatTek Corporation, Ashland, MA).
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Brain tissue for electron microscopy was prepared as previously described (Micsenyi et al., 2009).
T. reesei strains were incubated on solid rich-medium agar for 2 days, and mycelium for microscopy was prepared using the "inverted agar block" method (33).
The samples for transmission electron microscopy (TEM) and high resolution transmission electron microscopy (HRTEM) were prepared by dispersing small amounts of samples in ethanol to form a homogeneous suspension.
For scanning electron microscopy, samples were prepared essentially as described29.
The samples for transmission electron microscopy analysis were prepared by ultra cryo-microtomy using aLeica Ultracut UCT (Wien, Austria).
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