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Using excitation polarization-resolved second harmonic generation (SHG) microscopy, we measured SHG intensity as a function of the excitation polarization angle for type I and type II collagens.
Using confocal microscopy, we measured at the cellular level the growth of M. oryzae in the contrasted cases of Pi1 and Co39/Pia (see Methods).
By microscopy we measured internalization as the proportion of ligand-QD present inside individual cells expressing IR-B after different incubation times at 37°C.
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Using atomic force microscopy (AFM), we measured friction between an AFM tip and a polystyrene surface at 25 °C, as a function of the sliding velocity and the applied normal load, both in air and under vacuum conditions.
With fluorescence lifetime imaging microscopy (FLIM), we measured the temperature inside polymeric particles with high spatial resolution, which revealed increases in temperature upon irradiation at 980 nm.
By employing single particle tracking on the basis of fast video microscopy, we directly measure the equilibrium short and long time diffusion coefficients of blank and DNA-grafted (molecular weight of 250, 1000 and 4000 base pairs (bp)) tracer colloids in dilute λ-DNA solutions.
Given the high spatial resolution of PALM microscopy we could measure the dimensions of spines in great detail as revealed by intensity profiles across different dendritic structures (Fig. 2C).
Using the delivery of lipophilic dye by the Nanopatch™ (fluorescent DiD; imaged by confocal microscopy), Fig. 2m shows we measured the resultant distal tip penetration depth of 42±10 µm (mean ± SD; n = 350 projections, 5 ears) – extending through the epidermis (17.1 µm in thickness [21]) and into the upper dermis by 25 µm.
To illustrate the performance of this dual imaging microscopy system for biomedical imaging, we measured its axial resolution and imaged fixed and stained mouse retina.
Using low-energy electron microscopy (LEEM), we measure the growth rate of individual graphene islands and, simultaneously, the local concentration of C adatoms on the surface.
Using electron microscopy (EM), we measure the relative pH-dependent stabilities of the two different PA oligomers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com