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By means of Fluorescence microscopy we analysed the modifications of cell and nucleus architecture induced by the different substrata.
In order to further identify the viruses detected in queen ovaries by electron microscopy, we analysed a subset of the mated queen samples we received from various places in France (sampling A) for the presence or absence of 10 different honeybee viruses, using PCR-based assays.
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We characterized the in situ chromatin structure of each cell line by a biophysical approach: (1) we evaluated the rearrangements of the chromatin domains at the level of single cell by quantitative fluorescence microscopy; (2) we analysed the changes of the average chromatin condensation by differential scanning calorimetry.
Using immunofluorescence light microscopy of detergent extracted cells, we analysed cell lines containing a Ty epitope tagged copy of PFC3 at one of the endogenous alleles (18) and inducible RNAi against PFC3, PAR1 or PFC5 with the BB2 antibody.
From the results of X-ray diffraction and high-resolution transmission electron microscopy analyses, we conclude that the prepared nanocubes are truncated and contain (1 0 0) planes on six facets, with (1 1 1) planes appearing at the corners of the nanocubes.
Through X-ray diffraction in conjunction with micro-Raman spectroscopy and high resolution transmission microscopy analyses we have demonstrated that with the increase of nominal Li2MnO3 contents, the size of the ordered nano-domains (inside the active matrix) and the average size of the composite cathode particles increase systematically.
During the above microscopy analyses, we noticed that fluorescence intensities varied among different variants: those variants incorporated into chromatin generally gave bright fluorescence signals.
Based on our confocal and electron microscopy analyses, we hypothesize that ER-targeted ELP fusion proteins are synthesized on ribosomes associated with the rough ER and then transported into the ER lumen, where they accumulate and assemble into PBs by some unknown mechanism.
SAS performed Laurdan GP microscopy and analysed the data obtained.
Next, we performed electron microscopy (EM) analyses to assess ultrastructural changes in mitochondria caused by Trak1 depletion.
We recommend DRIFT microscopy for analyses of phytoliths extracted from soils because this technique allows measurements of selected, single siliceous phytoliths (as well as other BSi structures).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com