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Material for scanning electron microscopy was fixed and examined as explained previously [43].
Tissue for light microscopy was fixed in 10% phosphate-buffered formalin and embedded in paraffin.
Material for electron microscopy was fixed for 2 h at 4°C in 3% glutaraldehyde solution in buffer, pH 7.4.
Tissue for hematoxylin and eosin, periodic acid-Schiff and other special stains, as well as electron microscopy was fixed in 2% glutaraldehyde solution.
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Cells grown on 22-mm2 cover slips for conventional fluorescence microscopy were fixed with 4% paraformaldehyde for 12 h at 4°C.
Samples for electron microscopy were fixed in cacodylate-buffered glutaraldehyde (4.5%, pH 7.3), postfixed in osmium tetroxide (1%) and embedded in Spurr's resin.
Tissue samples for light microscopy were fixed in 4% formaldehyde and embedded in paraffin using routine procedures.
Parasitized erythrocytes for microscopy were fixed in methanol and stained for 10 minutes in 10 % Giemsa (Merck, Kenilworth, NJ, USA).
Cells used for immunofluorescence microscopy were fixed by 4% formaldehyde and 1 ml of cell suspension were treated by zymolyase T100 (100 μg), glucuronidase (30 μl) and 10 mM DTT for 30 min at 30°C.
Cells observed by electron microscopy were fixed with 2% glutaraldehyde and 4% paraformaldehyde (buffered with 10 mM cacodylate, pH 7.4) for 4 h and post-fixed with 1% osmium tetroxide for 3 h.
Samples for electron microscopy were fixed in 2.5% glutaraldehyde 0.1 M sodium cacodylate buffer, pH 7.4, overnight at 4 °C and processed for flat embedding in Epon 812 at the Facility for Electron Microscopy Research at McGill University (Montreal, Quebec, Canada).
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