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To identify isolate 76T-2, scanning electron microscopy was first performed.

To link the performance of the different LCM treatments with the varying physical properties of LC Ms,optical microscopy was first used to inspect sphericity and roundness of LCM particles (Fig. 8).

Auramine O fluorescence microscopy was first described by Hagemann in 1937 [3].

Three-photon (3P) microscopy was first demonstrated in the 1990s [ 17– 17].

Two-photon laser scanning fluorescence microscopy was first described by Denk and coworkers [ 27].

Fluorescence microscopy was first performed on two cancer cell lines (HT-29 and SW-480) to test and optimize the contrast agent.

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After 48 h, the slices processed for light microscopy were first incubated in biotinylated horse anti-mouse or goat anti-rabbit IgG (each at 1∶200 dilution, Vector Laboratories, Burlingame, CA, USA) for 2 h and then in avidin biotinylated horseradish peroxidase complex (Elite ABC, 1∶300 dilution, Vector) for 1.5 h, always at room temperature.

Transmission electron microscopy (TEM) was first applied to characterize the morphology and size of the SiNP ODN conjugates.

By fluorescence microscopy, NKX2-5 expression was first detected at day 4 of EB formation and peaked around day 7 (Fig. 2B).

Stimulated emission depletion (STED) microscopy was the first fluorescence microscopy technique to break the classic diffraction barrier of light microscopy.

CARS microscopy was demonstrated first by Duncan et al. in 1982 [ 20].

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