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The chemical composition and the success of hydrolysis have been assessed through infrared spectroscopy, while scanning electron microscopy verified that the spherical shape and the size (∼50 100 nm) of the NPs are practically unaffected by the hydrolysis process.
Drug uptake analysis tested by flow cytometry and confocal laser scanning microscopy verified that the uptake of FITC-UCCs-2/TPP nanoparticles was associated with urea transport protein on the membrane of H. pylori and reduced with the addition of urea as competitive transport substrate.
Field-emission scanning electron microscopy and transmission electron microscopy verified that the Li2MnSiO4/C nanocomposites finally obtained from 0.1 mol L−1 sucrose solution were agglomerates of Li2MnSiO4 primary particles less than 100 nm in size, and that there are thin carbon layers partially formed on the surface of these agglomerates.
Confocal microscopy verified that the target peptide was indeed binding to the adenoma in comparison with the control peptide in both the biopsy and ex vivo experiments.
Indeed, electron microscopy verified that staurosporine-treated Wt cells, but not DKO cells, exhibited hallmarks of apoptosis including nuclear condensation with maintenance of plasma membrane integrity, while ionomycin induced a purely necrotic phenotype that showed rupture of the plasma membrane and dispersion of the nucleus, which was not seen in DKO cells.
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Digital microscopy was used to verify that, at the end of washing process, the films resulted cleaned of residual FLG.
We selected representative samples for each treatment, examined them with light microscopy, and counted cells to verify that reduced DNA content connoted fewer cells.
Transmission Electron Microscopy (TEM) verified that cells of E. coli Bl21 were present in the intercellular space (Figure 2C) and inside cortex cells (Figure 2D).
Atomic force microscopy measurements verified that the sonication treatment successfully reduced the size and thickness of the GO nanosheets.
Transmission electron microscopy (TEM) verified that fibrils were still the predominant species present after a 2 h treatment with EGCG (Fig. 1B).
Statistical analysis of microscopy data (Fig. 5A, bottom panel) verifies that salubrinal is capable of significantly (p = 0.001) reducing the MG-132 induced ubiquitinated-protein accumulation in addition to protecting cells from ER stress, by interfering with protein folding and/or processing pathways.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com