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Samples were prepared for scanning transmission electron microscopy using a modified focused ion beam liftout procedure.
Direct counts of cells in suspension were performed by light microscopy using a Petroff-Hausser counting chamber.
Mitochondrial morphology was inspected by fluorescence microscopy using a dsRED filter on a Zeiss Axioskop microscope.
Cell movement was followed by phase contrast microscopy using a 10× objective.
Apoptotic nuclei were visualized by confocal microscopy using a Nikon Eclipse Te 2000-S microscope.
Cell labeling was monitored by fluorescence microscopy using a Nikon Eclipse E800 fluorescent microscope.
Coverslips were analyzed by conventional epifluorescence microscopy using a Zeiss Axio imager microscope.
The blue fluorescence was detected under fluorescence microscopy using a DAPI filter.
Cells were examined by standard fluorescence microscopy using a fluorescence microscope (Axioplan Microscope, Zeiss, Germany).
Samples were analyzed by confocal laser scanning microscopy using a Zeiss LSM 510 Meta confocal microscope.
Stained proteins were visualized by confocal microscopy using a FluoView™ laser scanning epi-fluorescence Olympus FV1000 microscope.
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