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In contrast to PALM and dSTORM, STED microscopy uses a specialized depletion laser, in combination with the excitation light.
Low magnification fluorescence microscopy uses a resolution of 500 µm to 1 mm and is used to monitor phenotypic effects on entire cell populations.
Intravital microscopy uses a wide variety of optical imaging techniques, often incorporating fluorescent or bioluminescent genetic reporters or markers, including nano-particles (Hoffman, 2005).
Since confocal microscopy uses a variable pinhole in front of the photomultiplier tube, light outside of the focal plane of the objective is blocked from detection.
Confocal microscopy uses a condenser to focus the light source within a small area of the cornea and an objective, which has the same focal point (hence the term confocal) as the condenser.
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Samples were prepared for scanning transmission electron microscopy using a modified focused ion beam liftout procedure.
Finally, the SERS spectrum of 4-ATP was analyzed by the Thermo Fisher Scientific DXR Raman microscopy using a 532-nm laser source.
Direct counts of cells in suspension were performed by light microscopy using a Petroff-Hausser counting chamber.
Mitochondrial morphology was inspected by fluorescence microscopy using a dsRED filter on a Zeiss Axioskop microscope.
Cell movement was followed by phase contrast microscopy using a 10× objective.
Apoptotic nuclei were visualized by confocal microscopy using a Nikon Eclipse Te 2000-S microscope.
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