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Moreover, this approach allows monitoring the growth of a single fibril using optical microscopy since the sensitivity of SHG microscopy to well-organized fibrils is below the optical resolution.
Here, we employ Raman spectroscopy and Raman microscopy to investigate well-defined oligomeric species: monomeric and dimeric analogs in solution, native T6 and R6 hexamers in solution and corresponding polycrystalline samples.
We performed confocal microscopy to assess expression levels as well as the subcellular localization of BST-2 following 8 h of IL-27 stimulation.
Here we introduce fluorescence and stimulated Raman scattering microscopy to quantify subcrystal reactivity as well as acid site distribution and to probe site accessibility in the set of individual mordenite zeolites.
Each emission was analyzed by light microscopy to sex the specimens, as well as to detect eventual contamination by somatic cells, and a total of 10 sperm and 10 egg samples were further analyzed.
Moreover, we showed that we could focus on the growth of a single isolated collagen fibril because SHG microscopy is sensitive to well-organized fibrils with diameter below the optical resolution.
In general, the morphologic pattern of innervation of mouse HCN4-positive cardiomyocytes identified using electron microscopy corresponds well to the dense network of nerve fibres demonstrated by fluorescent immunohistochemistry in mouse sinoatrial node and adjacent areas.
Electron microscopy is well suited to imaging gold nanoparticles due to their high electron density.
This approach calls for interdisciplinarity to be able to connect spectroscopy to ultrastructural and immunochemical analyses with e.g. electron microscopy, X-ray diffraction or atomic force microscopy as well as to biochemistry and molecular biology.
Our results demonstrate the value of hyperspectral two-photon for imaging gastric mucosa and also affirm that two-photon microscopy is well suited to resolving subcellular morphology in fresh GI tissues using only endogenous contrast [7].
The deficiencies in light compared to electron microscopy are well known.
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