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Then, the interface of the steel fibre was examined by the scanning electron microscopy to ensure the porosity in the vicinity.
The cultures were incubated for 26 hours to allow for schizont rupture and merozoite invasion (monitored by microscopy to ensure full schizont rupture).
The position of the pipette and bundle were continuously monitored before, during and after each recording using video microscopy to ensure that the stimulus pipette and the hair bundle moved in unison.
Four hours after plating the cells in 96 well plates, each well was checked by fluorescence microscopy to ensure that all fluorescent (NTERA2.Tom) cells were single cells and that no two fluorescent cells were closely situated to one another.
It was therefore necessary to perform confocal microscopy to ensure survivin's relocalisation within the cells.
Tissue morphology was assessed by phase contrast microscopy to ensure sufficient digestion of the collagen matrix.
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The morphology of the oligomers was investigated with transmission electron microscopy (TEM) to ensure that the change in pH does not induce oligomerization or disassemble the oligomers (Fig. S3).
Individual fibres were isolated from the bundles and examined by light microscopy (×100) to ensure their integrity and individuality.
Futhermore, we examined the virus via transmission electron microscopy (TEM) to ensure that the labeling procedure did not affect virion morphology.
Scanning electron microscopy was used to ensure that the porous structures of the scaffolds were preserved throughout the multiple conjugation steps, and Fourier transform infrared spectroscopy was used to monitor the reaction progress.
The mechanical properties (tensile strength, Young's modulus, peak load), metallurgical properties (porosity, wear, and material loss) supported with optical microscopy were investigated to ensure the recyclability of PA6 as a properties enhancing extrusion process.
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