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We combined AFM and fluorescence microscopy to demonstrate the functionality of proteins in the setup by documenting proton pumping by Halobacterium salinarium purple membranes.
The technique described suggest that diffusive labelling with vascular endothelial markers such as ulex europeaus agglutinin I in live tissue samples may be used in conjunction with confocal microscopy to demonstrate heterogeneous morphological and topological changes in intact segments of the microvasculature.
We used scanning and transmission electron microscopy to demonstrate that the obtained nanowires were fully porous, and this result was further supported by the fact that they were fully dissolved in an HF solution after successive HF and piranha treatments.
We then utilized whole mount confocal immunofluorescence (IF) microscopy to demonstrate that this maternal effect protein primarily localized throughout the egg and early embryo cytoplasm.
We also used biochemical approaches combined with fluorescence microscopy, to demonstrate that receptor endocytosis requires the integrity of specific membrane domains, namely lipid rafts.
We first used electron microscopy to demonstrate an increase in the number of clathrin-coated pits in HeLa or HEp-2 cells exposed to Stx.
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The electrochemical performance of the nanowire-based immunosensor array were characterized by (1) differential pulse voltammetry (DPV) to evaluate the specific recognition of PSA, (2) impedance and cyclic voltammetry to observe surface resistance and electroactivity, and (3) scanning electron microscopy (SEM) to demonstrate the three-dimensional architecture.
Here we use a multidisciplinary approach including subcellular membrane purification, fluorescence spectroscopy and Förster resonance energy transfer (FRET)/two-photon fluorescence lifetime imaging microscopy (FLIM) to demonstrate that initiation of vesicle fusion arises from two unique sites where these vesicles bind to chromatin.
To further demonstrate the induction of apoptosis by the peptide, we used fluorescence and electron microscopy (EM) to demonstrate apoptosis-associated morphological changes.
Ursell et al. [ 26 ] used specific labelling of overexpressed LamB (via a ybbr tag inserted into a surface exposed loop) in fluorescence microscopy experiments to demonstrate random, burst-like appearance of LamB on the surface of E. coli that moved to the poles during growth.
We therefore employed time lapse microscopy to directly demonstrate the serial killing of NK cells.
More suggestions(15)
microscopy to illustrate
scanning to demonstrate
microscopy to indicate
microscopy to ensure
microscopy to obtain
microscopy to verify
microscopy to monitor
microscopy to scrutinize
microscopy to identify
microscopy to pinpoint
microscopy to estimate
microscopy to examine
microscopy to assess
microscopy to investigate
microscopy to fix
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