Fluorescence recovery after photobleaching (FRAP) is a fluorescence microscopy method that enables the quantification of protein movement over time in living cells.
Here, we present the results of a study utilizing stereo-scanning electron microscopy (SSEM) that enables construction of digital elevation models (DEMs) of individual ash grains and calculation of bubble volumes from the remaining imprints using specialized software developed for this purpose (Proussevitch et al. 2011; Genareau et al. 2012).
The LSC system (CompuCyte Corporation, Cambridge, MA) is an automated microscopy platform that enables the measurement of protein biomarkers at the resolution of individual cells.
In this review, I describe light sheet fluorescence microscopy, a technique that enables rapid acquisition of three-dimensional images over large fields of view and over long durations, and I highlight recent applications of this method to microbial systems that include artificial closed ecosystems, bacterial biofilms, and gut microbiota.
JEOL and Nikon have integrated optical microscopy and field emission scanning electron microscopy in a way that enables seamless observation of the same region of interest on a sample with fast, accurate navigation.
To the Editor: Dermoscopy (also known as dermatoscopy, epiluminescence microscopy, and surface microscopy) is a noninvasive technique that enables rapid and magnified (×10) in vivo observation of the skin and detection of morphologic details often not visible to the naked eye.
To push the field further, cell biologists have begun borrowing machine-learning and data-mining tools from computer science, while engineers are designing imaging and microscopy systems that enable entirely novel analyses.
Here, Picco et al. developed a new fluorescence microscopy method that enabled them to track the positions of many of the proteins involved in endocytosis in yeast cells in real time.
This has been made possible through the use of a novel imaging system that enables confocal microscopy to be extended over large tissue volumes with preservation of image registration (40) as well as visualization tools that allowed us to standardize views with respect to local tissue microstructure and to separate perimysial collagen from surrounding myocardial tissue.
Two-photon excitation fluorescence microscopy is an advanced imaging technique that enables high-resolution observation of deep tissues due to the reduced scattering of high-intensity pulsed infrared laser light compared with conventional confocal microscopy [66,67].
