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Here, we describe a novel three-dimensional (3-D) dark-field microscopy that allows the real-time measurement of the number and spatial distribution of the injected Ba2+ ions throughout the microcapsules after treatment with sulphate.
This was made possible by the use of two-photon microscopy that allows brain imaging in living animals at a depth up to 1 mm.
Mieck et al. studied how a molecular motor made up of Kar3 and Cik1 moves along microtubules using sensitive microscopy that allows single molecules to be observed.
We suggest a compact coaxial design for optoacoustic microscopy that allows optimizing both the light illumination and ultrasonic detection parameters of the imaging system.
This is because AFM is the first and unique microscopy that allows high-resolution visualization of active biological samples in aqueous solutions.
γH2AX foci could be easily detected in cell nuclei using immunofluorescence microscopy that allows to use γH2AX as a quantitative marker of DSBs in various applications.
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We report on simultaneous application of SHG and CARS microscopy that allowed us to identify functional contractile sub-units of the skeletal muscle.
With the advent of electron microscopy that allowed visualization of nanometer scale structures [ 1] and advances in fluorescent dyes and light microscopy, it became evident that there is further sub-division and local organization within the nucleus and cytosol in the form of non-membrane bounded, macromolecular assemblies.
Therefore, we assessed random cell motility without external chemotactic stimuli using time-lapse microscopy that allowed assessment of migration rate as well as mean distance for individual cells as well as to observe the morphology of motile cells (Fig. 3; Kouvroukoglou et al. 2000).
MicroED is a recently developed method in cryo-EM (electron cryo-microscopy) that allows the collection of high-resolution electron diffraction data from extremely small three-dimensional crystals that are in the range of 0.1 0.4 µm thick (Shi et al., 2013 ▸) using a transmission electron microscope.
Photoactivated Localisation Microscopy (PALM) is a super-resolution microscopy technique that allows improving the spatial resolution of standard fluorescence microscopy by an order of magnitude.
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