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The chapter discusses the required instrumentation for FRET microscopy studies using either a steady-state or FLIM instrument.
Fluorescence microscopy studies using giant unilamellar vesicles (GUVs) as membrane model systems provide a unique methodology to quantify protein binding, interaction, and lipid solubilization in artificial bilayers.
Optical microscopy studies using crossed polarizers showed liquid crystalline mesophase in all the blends with PMPrS/PDHS=90/10 0/100.
Transmission electron microscopy studies using immuno-gold staining verified that transplanted mitochondria are internalized into cardiomyocytes [19, 20].
Through electron microscopy studies using wild type rice plants, OsHIR1 was found to mainly localize to the plasma membrane, with a minor portion localized to the tonoplast.
Our previous electron microscopy studies using thin plastic sections of mouse heart tissue have shown altered cristae morphology in the absence of LRPPRC (20).
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Two recent multiphoton microscopy studies used refined methods for identifying hematopoietic Ag sampling cells in the intestine 44, 45.
A recent electron microscopy study using flash-and-freeze technique revealed that in certain mutants of synaptotagmin 1 (with modified C2B region), the number of SVs located 0 5 nm away from presynaptic AZs transiently increased 10 ms after a presynaptic AP and decayed back with a time course similar to that of paired-pulse facilitation23.
Gorkun et al. correlated electron microscopy and turbidity studies using fibrinogen preparations missing one or both of these domains.
The paper by Abbott et al. (2012) uses light microscopy and finds lower densities of inhibitory synapses than other studies using electron microscopy.
This immunoelectron microscopy (IEM) study using magnetic beads was used to examine PCX-positive vesicles in urine samples from two patients (normoalbuminuric and microalbuminuric).
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