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See Supplementary Document for preparation of microscopy slides and immunofluorescence assay.
15 000 cells were seeded in 8-well microscopy slides and incubated for 24 h before replacing the medium with CellLight Early Endosomes-GFP at a concentration of 40 virus particles per cell.
Stained sections were mounted onto microscopy slides and visualized by confocal microscopy (Zeiss 510 META).
A small percentage of the larvae settled and metamorphosed on the microscopy slides, and those settled individuals were fixed for microscopy (as described below) after attachment.
One-third of the concentrated early-morning sputum pellet remaining after preparation of the microscopy slides and culture slants was frozen and shipped to the National Institutes of Health NIH Clinical Centerer, Bethesda, MD.
Coverslips were mounted on microscopy slides and confocal images were acquired on a Leica TCS SP5 microscope.
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Ten microliters of the suspension was applied to a microscopy slide and examined in a fluorescence microscope using a 495 nm filter, with a magnificence of 100×.
The vaginal swab was placed on a microscopy slide and analysed by Gram stain.
40 μl of suspended cells were immediately spotted onto a microscopy slide and visualized with a binocular microscope at 7x magnification (Olympus SZX16).
Polystyrene cuvettes (4 ml volume, Rotilabo, Carl Roth GmbH + Co. KG, Karlsruhe), which are otherwise commonly used for spectroscopic experiments, were glued onto microscopy slides for stability and filled with the tap water already used in the C. dubia culture.
Serial sections of 10 μm in thickness were deposited on microscopy slides for H&E and IHC staining.
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