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A persistent concern of using chemical fixation has also been the discrepancy between what electron microscopy sees and physiological experiments measure, particularly in terms of extracellular space.
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These different mosaic block configurations are directly imaged in real space by X-ray diffraction microscopy (see below).
Same aggregation between oppositely charged PEI wires was also evidenced by optic microscopy (see Additional file 1: SI-4).
Confirmation of the number and position of cells at the cantilever surface was obtained following experimentation using confocal scanning laser microscopy (see Fig. 1).
The vertical alignment of QDs was observed by transmission electron microscopy (see Figure 1) [6, 8]. Figure 1 Transmission electron micrographs of the cross section of the sample.
According to the data of scanning electron microscopy (see Fig. 1), the main part of the obtained material consists from two isolated fractions.
In the cross-sections and microscopical preparations isotropic glassy like particles with lemon yellow internal reflections, translucent with light yellow colour and very high relief were observed by means of optical microscopy (see Figure 3a).
In contrast to more rigorous models shown in literature [10, 23], this model does not include the effects of nanoparticle atomistic structure or effects of the presence of the vapor phase [24]; however, as seen from the results, it is still able to generate images of nanoparticles very similar to real images observed using atomic force microscopy (see Figure 1a-d).
To validate these predictions, we used a GFP-tagged Crz1 protein and fluorescence microscopy (see Materials & Methods).
Samples were fixed for microscopy (see below) at sequential time points including gametes (t = 0 h) and larvae at subsequent 24 h intervals until settlement.
(ii) In vivo detection of fluorescein-labeled dsRNA and morpholino oligos after transfection using fluorescence microscopy (see Fig. S5; e.g. [38]).
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