Slides were mounted using a drop of Fluoromount G (Electron Microscopy Sciences) and cells were visualized in a FLUOVIEW FV1000 confocal Olympus microscope.
About 80% of all microscopy investigations in the life sciences are carried out with light microscopy.
The historical sciences are sciences of understanding.
HeLa cells were grown in glass bottom dishes (Electron Microscopy Science), and live cell confocal microscopy was performed on Zeiss LSM 510 NLO META using a 20× objective.
Lyso-Tracker Red was purchased from Molecular Probes (Eugene, OR, USA); 4% paraformaldehyde/PBS was from Electron Microscopy Sciences (Washington, PA, USA); Texas Red-conjugated goanti-rabbitbIgGIgG were purchased from Cappel Research Products (Durham, NC, USA).
For Oil Red O staining, cells were fixed after 14 d with 10% v/v formalin solution, rinsed with PBS, and stained with Oil Red O (Electron Microscopy Sciences, Hatfield, PA), and adipocytes were imaged (Nikon Eclipse TS100 Inverted Microscope).
In brief, the CNT-AgNP thin film strip with silver paste (Silver Conductive Paint, 503, Electron Microscopy Sciences) applied on both ends was clamped into a dynamic mechanical analyzer (DMA) (Q800, TA instruments).
Formavar coated copper grids were from Electon Microscopy Sciences (Hatfield, PA).
EM reagents were from Electron Microscopy Sciences (Hatfield, PA) unless otherwise noted.
All the reagents and supplies for TEM were from Electron Microscopy Sciences (Fort Washington, PA, USA).
Eupergit C® was purchased from Röhm Pharma (Darmstadt, Germany) and glutaraldehyde (50%) was from Electron Microscopy Sciences (Fort Washington, PA, USA).
