Briefly, an aliquot of suspension were placed on a carbon-coated Formvar copper grid (300 mesh, Electron Microscopy Sciences) to form a thin film specimen.
The plastic coverslip with transfected cells were fixed by 2.5% glutaraldehyde (Electron Microscopy Science) for 1 h on ice.
In brief, the CNT-AgNP thin film strip with silver paste (Silver Conductive Paint, 503, Electron Microscopy Sciences) applied on both ends was clamped into a dynamic mechanical analyzer (DMA) (Q800, TA instruments).
After PBS washing, sections were incubated with Hoechst solution (4 µg/mL, Molecular Probes, Eugene, USA) for 4 min. After PBS washing, sections were mounted on Fluoromount-G (Electron Microscopy Sciences, Fort Washington, USA), sealed, and dried overnight.
Glutaraldehyde and osmium tetroxide were purchased from Electron Microscopy Sciences (Hatfield, PA, USA).
Glutaraldehyde and osmium tetroxide were purchased from Electron Microscopy Sciences (USA).
A carbon-coated copper TEM grid was purchased from Electron Microscopy Sciences (Hatfield, PA).
PFA was purchased from Electron Microscopy Sciences (Hatfield, PA).
Formavar coated copper grids were from Electon Microscopy Sciences (Hatfield, PA).
Paraformaldehyde (PFA) was from Electron Microscopy Sciences (Washington, PA).
Paraformaldehyde was purchased from Electron Microscopy Sciences, USA.
