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Cryoelectron microscopy reconstructions of free and DNA-bound forms of the Cascade/I-C surveillance complex reveal conformational changes that enable R-loop formation with distinct positioning of each DNA strand.
Epitope mapping of the neutralizing monoclonal antibodies (mAbs) indicated major contacts with the V2 apex, and 3D electron microscopy reconstructions of Fab-trimer complexes revealed a horizontal binding angle to the Env spike.
Based on this new structure, we reanalyzed electron microscopy reconstructions of UvsX DNA filaments and docked the UvsX crystal structure into two different filament forms: a compressed filament generated in the presence of ADP and an elongated filament generated in the presence of ATP and aluminum fluoride.
Interactions between actin subdomain 1 and TM in relaxing conditions were suggested by electron microscopy reconstructions of the regulated thin filaments (22, 23).
(A ) Cryo-electron microscopy reconstructions of COPII cages assembled in vitro in the presence of inner coat subunits, but in the absence of a membrane, showed additional density below that of the outer coat.
Electron microscopy reconstructions of serial sections of skeletal muscle dating back over 50 years presented a puzzle: the thin/thick filament arrangement in the sarcomere alters its geometry, progressing from a tetragonal lattice arrangement at the Z-band to a trigonal arrangement in the A-band (Pringle, 1968), with the transition taking place near the I-A junction (Traeger et al., 1983).
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Here, we report cryoelectron microscopy reconstruction of a functional Cmr complex bound with a target RNA at ∼12 Å.
Here, we determine a 7.1 Å cryo-electron microscopy reconstruction of the bacteriophage-encoded PhuZ filament and provide molecular-level insight into its cooperative assembly mechanism.
Here, we present the crystal structures of the iron-free and iron-loaded frataxin trimers, and a single-particle electron microscopy reconstruction of a 24 subunit oligomer.
Despite their linkage to human mitochondrial diseases and the recent cryoelectron microscopy reconstruction of yeast and mammalian mitoribosomes, how they are assembled remains obscure.
We also present an 18 Å cryo-electron microscopy reconstruction of the hexameric MCM from Methanothermobacter thermautotrophicus, and fit the atomic resolution crystal structure into the reconstruction in order to generate an atomic model for the oligomeric assembly.
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