Suggestions(1)
Exact(1)
For microscopy, plants were grown almost vertically on MS plates supplemented with 1% (wt/vol) sucrose and 0.5% (wt/vol) Phytagel (Sigma Aldrich, St . Louis MO, P8169).
Similar(59)
Epifluorescent microscopy of plants was performed using the tdTomato filter set: 535/30 nm excitation and 600/50 nm band pass emission or the mOrange filter set: 535/30 excitation and 585/40 nm band pass emission filter (Olympus stereo microscope model SZX12, Olympus America, Center Valley, PA, USA).
The roots of the transgenic plants were visualized by fluorescence microscopy.
Epidermal cells of exo and wild-type plants were analyzed using scanning electron microscopy (SEM) and found to be smaller in the exo mutant.
All plants were grown as described above for microscopy.
Reproductive organ development and fertilization in these plants were examined by various approaches, including histological analysis, scanning electron microscopy, transmission electron microscopy, and phenotypic analyses of reciprocal crosses between wild-type and transgenic plants.
Western analysis was first used to screen for plants expressing high levels of GFP, and selected plants were then analyzed by confocal laser scanning microscopy (CLSM).
The roots of ten-day-old transgenic plants were visualized by differential interference contrast (DIC) and fluorescence microscopy.
Plants were carefully removed from the medium and roots washed with deionized water before being analyzed by confocal laser microscopy (CLSM, see details below).
Plants were washed once with 70% (v/v) ethanol, destained in 70% ethanol for 16 h and observed by light microscopy [64].
The internal structure of pericarp and sepals from both wild type and transgenic plants was examined by light microscopy.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com