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To determine the morphology and the size of the resulting nanoparticles, transmission electron microscopy pictures were taken on a PHILIPS CM120 electron microscope operating at an accelerating voltage of 120 kV.
Light, DIC and fluorescence microscopy pictures were taken using a Zeiss Axioskop 2 Plus Fluorescence Microscope mounted with a AxioCam MRM digital camera, or alternatively with a Nikon Eclipse E400 microscope and a Nikon CoolPix 990 digital camera.
The surface morphology of emulsion droplets was observed using an optical microscopy; pictures were taken using a digital camera.
All EM (electron microscopy) pictures were prepared and imaged at the University of Chicago Electron Microscopy Core.
For confocal microscopy, pictures were assessed in a Zeiss 510 Meta microscope and analyzed using the Zeiss LSM Browser 4.0.0.157 software, at the PICsL (Plateforme d'Imagerie Commune du Site de Luminy) facilities.
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Nomarski contrasted, bright-field microscopy pictures are indicated as BF.
Note that the fuzziness of the cryo-immunogold electron microscopy pictures is owing to the mild fixation.
The median size of the isolated MVs, as observed in multiple electron microscopy pictures, was 422 nm, with only 3.4% of the MVs below 0.1 μm and 0.4% above 1 μm.
Cells were fixed in 96% ethanol, stained with hematoxylin and eosin (Merck, Germany), and light-microscopy pictures were taken (Leica DMIL LED, LAS-EZ 2.0, Germany).
As a reference, a bright field transmission microscopy picture was taken.
A bright field transmission microscopy picture was taken as a reference.
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