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Nomarski contrasted, bright-field microscopy pictures are indicated as BF.
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To determine the morphology and the size of the resulting nanoparticles, transmission electron microscopy pictures were taken on a PHILIPS CM120 electron microscope operating at an accelerating voltage of 120 kV.
Light, DIC and fluorescence microscopy pictures were taken using a Zeiss Axioskop 2 Plus Fluorescence Microscope mounted with a AxioCam MRM digital camera, or alternatively with a Nikon Eclipse E400 microscope and a Nikon CoolPix 990 digital camera.
All EM (electron microscopy) pictures were prepared and imaged at the University of Chicago Electron Microscopy Core.
The surface morphology of emulsion droplets was observed using an optical microscopy; pictures were taken using a digital camera.
Note that the fuzziness of the cryo-immunogold electron microscopy pictures is owing to the mild fixation.
For confocal microscopy, pictures were assessed in a Zeiss 510 Meta microscope and analyzed using the Zeiss LSM Browser 4.0.0.157 software, at the PICsL (Plateforme d'Imagerie Commune du Site de Luminy) facilities.
The median size of the isolated MVs, as observed in multiple electron microscopy pictures, was 422 nm, with only 3.4% of the MVs below 0.1 μm and 0.4% above 1 μm.
As a reference, a bright field transmission microscopy picture was taken.
A bright field transmission microscopy picture was taken as a reference.
Cells were fixed in 96% ethanol, stained with hematoxylin and eosin (Merck, Germany), and light-microscopy pictures were taken (Leica DMIL LED, LAS-EZ 2.0, Germany).
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