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Possible applications are in beam-forming design using high-aperture or collimated cylindrical laser/electron quasi-Gaussian beams in imaging microscopy, particle manipulation, optical tweezers, and the study of the scattering, and radiation forces on objects.
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Particle manipulation by acoustics has been investigated for many years.
Laminar flow, hydrodynamic particle manipulation has been extended to micropost arrays.
Atomic force microscopy enables manipulation and characterization of individual molecules at atomic resolution.
Apart from generating focal hole with long focal depth, it is observed that a properly designed CPM also generates multiple focal hole segments useful for laser cutting, microscopy and the manipulation of optical traps of low refractive index particles.
Szymborska, A. et al. Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging.
Finally scanning electron microscopy allows particles measurement [ 68, 69].
Transmission electron microscopy demonstrated spherical particle morphology.
Because single cell analysis is usually performed under microscopy, the micro-nanorobotic manipulation has to be workable under microscopy.
For all electron microscopy imaging techniques, particles were imaged as-is, with no further preparation.
The new technology combines traditional optical microscopy with tiny particles, called transparent microspheres, that magnify images.
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