After another three washes, slides were mounted with mounting medium with DAPI (Vector Laboratories, UK) and analyzed by fluorescence microscopy on a DMI3000B microscope (Leica Microsystems, UK).
Subsequent immunofluorescent co-staining was performed, and images were obtained by confocal microscopy on a Nikon A1R LUN-V and analyzed on Nikon Elements.
Dispersion of SWNTs in these nanocomposites was characterized by optical microscopy on a micrometer scale.
Images of nuclei were obtained by inverted fluorescence microscopy on a BX53 microscope (Olympus, Shinjuku-ku, Tokyo, Japan) equipped with a DOC-CAM HR CCD camera (Molecular Devices, Chuo-ku, Tokyo, Japan).
Epifluorescence images were acquired by spinning disk microscopy on a customized microscope setup as previously described60,61,62 except that the system was upgraded with a next generation scientific CCD camera (cMyo, 293 Photometrics) with 4.5 μm pixels allowing optimal spatial sampling using a ×60 NA 1.49 objective (CFI 294 APO TIRF; Nikon).
The morphology of powders was recorded using Field Emission Scanning Electron Microscopy on a Mira3-XMU system.
Fixed specimens were scanned at the Duke Center for In Vivo Microscopy on a 9.4 Tesla magnetic resonance system adapted explicitly for microscopic imaging.
They're also intending to offer a high tech alternative to carrying out manual microscopy on a blood smear — another technique that can be used to conduct an point-of-care CBC test, but which requires specialist personnel taking the time, care and attention to get it right.
The sections were stained with toluidine blue and examined by differential interference contrast microscopy on a Zeiss Axioplan 2 imaging light microscope (Zeiss, Jena, Germany) equipped with a DP70 Olympus camera (Shinjuku, Tokyo, Japan).
The effects of different laser parameters are analyzed by using a combination of advanced sample preparation and ultra-high resolution scanning electron microscopy on a nanoscale level and a specific combination of mechanical tests on the macroscopic level.
