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Fluorescence microscopy of yeast was performed with a Leica DM4000B microscope.
Fluorescence time-lapse microscopy of yeast cells expressing GFP-tagged Nrp1 from the endogenous locus.
(A ) Fluorescence microscopy of yeast cells expressing sfGFP-tagged Rnq1PD in [ PIN+ ] and [ pin− ] cells.
Indeed, fluorescence microscopy of yeast P-bodies revealed a smooth spherical surface, in agreement with a liquid-droplet state.
Fluorescence microscopy of yeast cells expressing Pbp1-GFP from the endogenous locus and mCherry-tagged Ubc9ts from a plasmid.
Immunofluorescence microscopy of yeast cells with anti-Ald4p antibody clearly showed that anti-Ald4p antibody specifically binds to MFIBs (Fig. 3B).
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Despite their linkage to human mitochondrial diseases and the recent cryoelectron microscopy reconstruction of yeast and mammalian mitoribosomes, how they are assembled remains obscure.
(B ) Z-projections of confocal fluorescence microscopy images of yeast cells expressing mito-DsRed and Mic60-EGFP expressed from its endogenous locus in the indicated yeast cells.
The FATC domain has also been interpreted to have been visualized in low resolution electron microscopy structures of yeast Tor1 and human DNA-PKcs; in these structures, it is shown to protrude from the KD (Rivera-Calzada et al, 2005; Spagnolo et al, 2006; Adami et al, 2007).
Fluorescence microscopy of WSP1267-treated yeasts incubated with Nile Red revealed an accumulation of lipid droplets in the cytoplasm of treated yeasts when compared to C. albicans (isolate 77) control, indicating that WSP1267 can induce the accumulation of lipids and/or precursors.
Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast.
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