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Both rBCGs were clearly imaged and easily quantified with ultraviolet microscopy of tissue sections and whole organ suspensions.
Migrating cells were clearly observed at the cartilage/scaffold interface using fluorescence microscopy of tissue cryosections (Fig. 4B and C).
Centrosomes were visualized in the glioma samples using confocal microscopy of tissue sections to visualize γ-tubulin and hNinein expression (Fig. 2B).
Cryosections (150 μm thick) of tumors and lungs were prepared and imaged as described in the Extended Experimental Procedures section "Immunostainings and Confocal Microscopy of Tissue Sections".
The tumor selectivity of Cu-porphysomes was further demonstrated by fluorescent microscopy of tissue slices, comparing tumor and uninvolved prostate tissue from the orthotopic PC3 and 22RV1 models with healthy prostate from surgery-only mice.
Oral ingestion of YTX at doses as high as 2 mg kg−1 has not led to mouse death and detection of severe organ damage (Aune et al, 2002; Tubaro et al, 2003), but ultrastructural alterations of cardiomyocytes have been observed by electron microscopy of tissue samples from mice that ingested high YTX doses (Tubaro et al, 2003).
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Organic phosphors are used as optical brighteners for invisible markers of laundry, banknotes, identity cards, and stamps and for fluorescence microscopy of tissues in biology and medicine.
Novel tools in the 3D nuclear imaging arsenal now include high-throughput scanning capabilities and new 3D nano-resolution microscopy of tissues and cells.
A key challenge in optical spectroscopy and microscopy of tissues lies in decoupling their absorption and scattering signals [ 6].
Pulmonary artery remodeling was assessed using confocal microscopy of tissues stained with a nuclear dye (DAPI) and CD11b antibody.
BBB score was recorded which increased significantly along with the reduction in cavity area observed by bright field microscopy of tissues, that is, from 1 to 10 and from 0.20 ± 0.05 mm to 0.10 ± 0.03 mm, in Sal B treated group, respectively, compared to PBS group.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com