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Using fluorescence microscopy of skin sections, the green fluorescent AHAPS-SiO2-NP were localized only in the superficial layers of the stratum corneum in all particle-treated mice as shown earlier [17].
Finally, we also assessed the effect of the depletion of the anti-p170 antibodies by immunofluorescence microscopy of skin cryosections using the PNP serum no. 9 with strong and apparently selective reactivity with p170 (Figure S4).
GC and VP performed transmission electron microscopy of skin biopsy.
Microscopy of skin sections containing epidermis showed compact keratinization, parakeratotic foci, and irregular hyperplasia with a pseudoepitheliomatous area.
Loss of expression of the targeted loci was confirmed by immunoblotting of neonatal skinderived mast cells and/or immunofluorescence microscopy of skin mast cells.
Another study evaluating the effect of microinjections of a vitamin/HA solution by histology and electron microscopy of skin biopsies also failed to show changes in epidermal and dermal thickness [ 4].
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Confocal microscopy images of skin exposed to nanoparticles have therefore been assessed by quantitative statistical analysis.
Prerequisites for selection were that clinical signs compatible with Cheyletiella infestation were verified by a demonstration of Cheyletiella mites through light microscopy examination of skin scrapings, material from flea combing or transparent tape preparations under 4 or 10 × 100 magnifications.
Electron microscopy of the skin samples from the proband showed that the skin separation localized to the base of the basal keratinocytes (Fig. 2D).
Confocal laser microscopy of rat skin showed that optimized formulation was eventually distributed and permeated deep into the skin.
Confocal laser microscopy of rat skin treated with sodium fluorescein labeled NLC showed a significantly (p < 0.001) deeper penetration (187.34 μm) as compared with the conventional gel (19.25 μm).
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