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Light microscopy of deletion strains.

Electron microscopy of P. pastoris deletion mutants grown on YPD or YPO did not show altered organelle structure.

(A and B ) Electron microscopy of wild type and AIM24 deletion mutant.

By constructing a series of deletion mutants fused with EYFP and fluorescence microscopy analysis, we identified an N-terminal arginine-rich functional NLS (PASTPTPPKR GRYVVEHPEY) in P-ICP22, and KR is the crucial aa for its nuclear localization.

Analysis of wild type (WT), apt1Δ (mutant) and apt1Δ::APT1 (complemented) strains by transmission electron microscopy revealed that deletion of APT1 resulted in the formation of irregular vacuoles.

Nevertheless, no shared region of deletion has been identified among the autistic patients with 18q deletion.

Three alleles of qSW5/GW5 gene were distinguished by length of deletion at the qSW5/GW5 locus (Yan et al. 2011): Kasalath-allele (no deletion), indica II-allele (950 bp deletion), and Nipponbare-allele (1,212 bp deletion).

Figure 3 Growth of deletion strains in different carbon sources.

Use of deletion constructs resulted in loss of inducibility.

Live cell microscopy of Kar3-3xGFP (green) in either wild-type (upper panel) or bim1-deletion mutant (lower panel).

Additional file 1: Figure S2 shows light microscopy images of the deletion strains.