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Fig. 2 Scanning electron microscopy of cells cultured on various nanoporous stainless steel artificial microenvironments.
Furthermore, immunoelectron microscopy of cells transfected with the knockout bacmids revealed defects in nucleocapsid production for all three constructs.
Here, we describe recent progress in electron microscopy of cells and the ways in which the relevant methodologies are helping to elucidate cell architecture.
Fluorescence microscopy of cells labeled with Gd-DOTA-k FR -Gal-CPP indicated a predominantly vesicular localization of the Gd-DOTA-k FR -Gal-CPPjuGd-DOTA-k FR -Gal-CPPei.
The tilt series data were treated for image processing and reconstruction using the IMOD software program from Boulder Laboratory for 3D Electron Microscopy of Cells and the University of Colorado [26].
In the present study, all five strains of Y. lipolytica when grown in 30 g L -1 glucose revealed a variable number of LBs which could be visualized by light microscopy of formaldehyde fixed cells or by fluorescence microscopy of cells stained with Nile red (Figure1 insets).
At the Boulder Laboratory for 3D Electron microscopy of cells, methods are developed for high resolution, large area ET of thick, stained plastic sections for the 3D reconstruction and analysis of mammalian membrane architecture at high fidelity in the insulin-secreting beta cells of the endocrine pancreas.
Infection efficiency was shown to be >90% by fluorescent microscopy of cells infected with Ad5-GFP (24 h).
Localization of newly replicated DNA was determined by immunofluorescence microscopy of cells immunostained with purified SeqA antiserum.
Confocal microscopy of cells transfected with bestrophin-1 and β3-subunits showed a co-localization of the two proteins which was however more uniformly distributed in the cytoplasm.
Deconvolution confocal microscopy of cells fixed at 24 h p.i. revealed a capsid distribution significantly different from that of NS1-deYFP.
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