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These were also confirmed by the scanning electron microscopy of CLEAs papain (Fig. 5).
Similar(59)
The size of various CLEAs (and their clusters) has been reported to be in the range of 0.1 μ to 100 μm [7]. Figure 3 Scanning electron microscopy (SEM) image of CLEA: SEM was carried out on Zeiss EVO50 scanning electron microscope, UK.
Nano-TiO2 addition decreased Km and increased Vmax of CLEAs.
The activity of CLEAs was tested by using an appropriate aliquot of CLEAs that guaranteed a linear response.
Since the content of PP is assumed to be constant all throughout the mass of CLEAs recovered, the mass of PP present in the aliquot of CLEAs assayed could be simply obtained once the ratio of the mass of CLEAs assayed to the total mass of CLEAs recovered was known (Columns "Mass of CLEA assayed (aliquot)"/"Total mass of CLEAs recovered" in Table 3).
These changes were benefit to the activity and structure of CLEAs.
Under the optimum preparation conditions, the activity recovery of CLEAs recorded 92%.
Intermediate calculated values needed for the determination of the retained activity of CLEAs have been summarized.
The efficient decolorization (> 90%) of remazol brilliant blue R and slight decrease in CLEA activity were measured over a 10 h period of continuous operation, which illustrates the potential of CLEAs for the wastewater treatment.
This was possibly due to more channels and smaller size particle of CLEAs after nano-TiO2 addition.
The use of CLEAs from various enzymes – proteases, amidases, lipases, nitrilases, nitrile hydratases, hydroxynitrile lyases – in enantioselective syntheses is described.
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