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The cell-to-cell variation of GFP expression is easily visible in fluorescence microscopy (note that the FACS and imaging data are not directly comparable because the linearity properties of the two techniques are quite different).
To validate these rules, we first collected experimental data describing growth characteristics of co-cultured HMEC and vHMEC imaged by time-lapse microscopy (note: vHMEC were obtained by taking HMEC that had escaped stasis in a previous experiment).
Then, mechanically extended chromatin fibers were prepared from these cells and examined by IF microscopy (note that the term "fiber" is used throughout this study to refer to chromatin preparations; in contrast, the term "thread" is used to designate the PICH-positive structures seen in anaphase cells in situ; Baumann et al. 2007).
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Whereas we cannot definitively assign the SDS-stable Sup35 NM aggregates detected by filter retention to those structures detected by fluorescence microscopy, we note that fluorescence microscopy of prion-containing yeast cells has also revealed structural diversity (Derkatch et al., 2001; Zhou et al., 2001).
While imaging these cells by confocal microscopy, we noted that in some cell types the constructs displayed a slight tendency to form highly fluorescent aggregates (speckles) in a fraction of the cells (Figure 5).
We observed the morphological changes by microscopy and noted that the chlamydial developmental lifecycle lasted ∼27 h in THP-1 monocytes, which is similar to that of HeLa cells.
Using confocal microscopy we noted that syndecan-1 was localized within the nucleus of CAG myeloma cells expressing low levels of heparanase (HPSE-low cells) but it not present within the nucleus of CAG cells expressing high levels of heparanase (HPSE-high cells) (Fig. 1).
Based on observations by light microscopy, it was noted that samples collected at the edges of the mat contained more sheathed cells than deeper layers, and that the sheaths appeared less mineralized.
By confocal microscopy we also noted a punctate or speckled RBM45 nuclear staining in neurons containing RBM45, TDP-43 or ubiquitin inclusions (Figs. 5, 6, 7).
Note Raman microscopy was unable to identify these variations.
Of note, electron microscopy revealed diffuse foot process effacement.
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