By electron microscopy it has since long been known that in mammals, nuclear condensation takes place following a gradient from cranial to caudal (Courtens et al., 1995; Courtens and Loir, 1981; Dooher and Bennett, 1973).
Furthermore, the limited imaging depth of fluorescence microscopy also places restrictions on clinical application.
4, 6, 7 As compared to conventional imaging, super-resolution fluorescence microscopy places higher demands on the fluorescent probes in terms of brightness, photostability, and in some cases the requirement for photo-switchable fluorescence emission.
If the present observations are validated in larger multicentre, clinical trial, the test may prove to be a useful alternative to microscopy, particularly in places where the facilities for microscopy are poor or non existent.
Complementary subcellular localization approaches including nitrogen cavitation coupled to Percoll fractionation, immunofluorescence, confocal and electron microscopy collectively placed Nuc, COX-2, and all of the main enzymes involved in prostanoid synthesis, in the Golgi apparatus and endoplasmic reticulum of human neutrophils.
Myxospores from Striped Marsh frog's gallbladder were prepared for scanning electron microscopy (SEM); placed on poly-L-lysine coated coverslips, fixed in 1% OsO4 in 0.2 M sodium cacodylate buffer (pH 7.0), rinsed in distilled water, dehydrated with a graded acetone series, critical point dried, and coated with gold.
The density of deposited spores (per mm) was estimated on glass microscopy slides, placed in the settling tower.
