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Light microscopic evaluation of conjunctival swabs may be a useful minimally invasive first step toward diagnosis of microsporidial keratoconjunctivis in settings where electron microscopy is not available.
Digital microscopy is not new - tissue scanning first appeared a decade ago, replacing the conventional method of manually cutting ultra-thin slices of tissue one by one to then examine them under a microscope.
The resolution of conventional electron microscopy is not limited by electron wavelength, but by the large aberrations of electron lenses.
Indeed, there is critical concern when high-resolution confocal or deconvolution microscopy is not used to track cell lineage in allografts (see editorial by Hoofnagle et al.[11]).[11]
Since the anti-BLM serum appropriate for confocal microscopy is not available, the presence of Dd-BLM conjugate was demonstrated using the anti-Dd antibody.
The likely role of MR microscopy is not to supersede the utility of light microscopy, but to add intriguing capabilities beyond and complementary to those of light microscopy.
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Fourier transform infrared spectroscopy microscopy was not carried out here.
2D images from scanning or transmission electron microscopy are not sufficient for this purpose but a three dimensional (3D) reconstruction of the membranes is required.
We found that Pt nanoparticles, characterized by transmission electron microscopy, are not perfect cubo-octahedrons and that large particles have "rougher" surfaces than small particles.
Furthermore, with this letter, we hope to help other researchers to realize that TEM using the drying method as well as scanning electron microscopy or atomic force microscopy are not appropriate techniques for determining the morphology of any soft-matter species [2 4].
The mean number of persons examined by smear microscopy was not different.
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