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Localization of specific structures and imaging of cellular dynamics in cellular cryo-electron microscopy is limited by (i) the use of cryo-fixation to preserve cellular structures, (ii) the restricted availability of electron dense markers to label molecules inside cells and (iii) the inherent low contrast of cryo electron microscopy.
The resolution power of conventional optical microscopy is limited by light diffraction.
The resolution of conventional fluorescence microscopy is limited to ∼200 nm in lateral (x, y) dimensions, and ∼500 nm along the optical axis.
Conventional confocal microscopy is limited by the depth of tissue that can be examined, restricting its use to the earliest stages of embryonic development.
Nevertheless, only the largest cellulose bundles could be visualized, as the resolution of conventional light microscopy is limited to around 250 nm in xy-direction.
While revolutionary for its ability to visualize the activity of neuronal populations interacting with each other, confocal microscopy is limited by its penetration depth and the use of exogenous voltage sensitive dyes.
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Previous studies measuring cAMP in phagocytes with biochemical assays and fixed-cell microscopy were limited in their temporal resolution.
Further reasons for possible under-diagnosis of TBM were that not all CSF samples were sent for TB studies, volumes of CSF examined were low (typically 1-2 mls), and time for smear microscopy was limited.
As fibre cell length can often be greater than 1 mm (and sections for microscopy were limited to 25 μm depth) efforts were made to compare these partial cell images to 3D data.
However, TIRF-Microscopy is limited to the visualization of vesicles that are located near the membrane attached to the glass coverslip on which the cell grows.
In areas where malaria is less prevalent, or very rural areas where access to good quality microscopy services is limited, RDTs may be less expensive than microscopy (WHO 2008).
More suggestions(15)
light is limited
pass is limited
scan is limited
scanner is limited
microscopy is restricted
sweep is limited
microscopy is indicated
microscopy is reported
microscopy is composed
microscopy is presented
microscopy has limited
microscopy is captured
microscopy is described
microscopy is used
microscopy is demonstrated
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