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The surface morphology of R. stricta activated carbon as obtained by scanning electron microscopy is given in Fig. 2.
The relevant theoretical evaluation of reflection contrast microscopy is given by Bereiter-Hahn et al. and Gingell and Todd [ 11, 12].
For the calculations, we used the same parameters that were used in Fig. 2. The effective PSF for SAX microscopy is given by the product of a detection PSF and a PSF of demodulated fluorescence [ 12, 14].
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Details of fixing, staining and microscopy are given in Methods S1.
The key microscopic characteristics of the five powdered crude drugs under normal and polarized light microscopy are given in Fig. 2 and Fig. 3.
A further two patients negative in both the breath test and by smear microscopy were given a final diagnosis of tuberculosis, one of whom (P30) admitted during further investigation that he had previously received treatment for pulmonary tuberculosis and was classified as an immunocompromised re-treatment case.
Detailed information on microscopy data is given in Additional file 2. Exposition of A549 cells to 9.2 × 10 particles mL−1, corresponding to mass concentrations of 1.2 μg SiO2 mL−1 (25 nm SNPs) and 50 μg SiO2 mL−1 (85 nm SNPs), was not found to exert a detectable influence on cell morphology, regardless of the medium composition.
Evidence of the CNS formation is given by optical microscopy, scanning electron microscopy, and transmission electron microscopy.
The most important stages of the analysis involve microscopy, an overview of which is given, and detailed information on optical stereo, optical compound and scanning electron microscopy is presented.
Efficiency of transfection was assed qualitatively by fluorescent microscopy of which an example is given in Fig. 1a.
Assessment of percentages of positive cells was performed across the entire sample in a blinded fashion upon light microscopy by two investigators and is given as the mean of both percentages assessed in steps of 5%.Percentages of staining refer to epithelial/tumour cells within samples.
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