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Here, the strategy for engineering novel nanocarriers for controlled nucleus-targeted antitumor drug delivery and real-time imaging by single- or two-photon microscopy is described.
The protocol for the staining of FFPE material for confocal microscopy is described elsewhere [34], [41].
Our technique for in vivo microscopy is described in detail elsewhere [ 24] (video footage illustrating the technique is available on the internet [ 25]).
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The design, capabilities, and limitations of confocal microscopy are described along with some guidelines for best practices in using confocal microscopy.
The method involving type X collagen immunostaining and confocal laser scanning microscopy was described above.
Tissue preparations for microscopy are described below.
Details of microscopy were described previously (Pidoux et al, 2000, 2003).
The system lay-out and properties (for reflectance microscopy) are described in detail elsewhere [ 32].
28 Double and triple immunolabeling for light and confocal fluorescence microscopy are described in the Supplementary Materials and Methods.
Microwave fixation and processing of cell monolayers for transmission electron microscopy was described in detail elsewhere [43].
In situ processing technique of cultured cells in 6-well plate for the transmission electron microscopy was described previously.
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