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Maiden, A. M., Humphry, M. J. & Rodenburg, J. M. Ptychographic transmission microscopy in three dimensions using a multi-slice approach.
Two acid-fast bacilli per visual field were detected by direct microscopy of a spontaneously produced sputum specimen, and acid-fast bacilli were also detected by microscopy in three induced sputum and one bronchoalveolar lavage specimens.
With new techniques [ 120], such as stochastic three-dimensional optical reconstruction microscopy, STORM, a resolution can be obtained which enables the visualization of cellular structures by light microscopy in three dimensions.
Electron microscopy has been used to probe different genetic conditions (Acebes and Ferrus, 2001; Acebes et al., 2011) but could not definitively assign glomerular identity or cell type for individual synapses, highlighting the need for structural analysis at the level of light microscopy in three dimensions.
The total number of TUNEL-positive cells and DAPI counterstaining-positive cells was counted individually using fluorescence microscopy in three slides from each brain, with each slide containing three fields from the ischemic boundary zone and one field from the ischemic core.
Although the positive results of the S. stercoralis real-time PCR was confirmed by microscopy in three cases only, the other positive results were supported by serology (n = 7), eosinophilia (n = 5) and/or clinical presentation (n = 4) in the majority of cases.
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Bethge, P., Chéreau, R., Avignone, E., Marsicano, G. & Nägerl, U. V. Two-photon excitation STED microscopy in two colors in acute brain slices.
In this study, carbon-based black pigments were identified by Raman microscopy in four Jesuit wooden sculptures produced in the 18th century.
The number of immunoreactive cells in each well was counted using fluorescent microscopy in four independent fields.
It differs from fluorescence microscopy in two major factors: light source and a pinhole.
Interestingly, the sensitivity of phage based assays was lower than that of microscopy in four studies [ 8, 9, 13, 15].
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Justyna Jupowicz-Kozak
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