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As graphene is replete with unique structural and electronic properties scanning probe microscopy has proved to be an exciting and a rewarding venture.
In physiological systems, Laurdan microscopy has proved its usefulness in showing distinct clustering of Caveolin-1 with membrane domains of increased order [34] and has shown the increased order of the lamellopodia of macrophages [35] compared to other regions of the cell's membrane.
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Cross-section microscopy has proven to be highly complementary with MA-XRF, combining the overall compositional information of a painting with local, but layer specific analysis of a paint system on the microscale [5].
THG microscopy has proven effective for studying cell divisions in the early zebrafish embryo [ 5].
One technique, Laurdan fluorescence microscopy, has proven very useful for distinguishing such regions but has hitherto relied on 2-photon confocal microscopy.
Serial block-face scanning electron microscopy (SBEM) has proved to be a remarkable technique for imaging at moderate lateral and axial resolution (approximately 10 and 40 nm, respectively) and across large fields of view spanning many hundreds of microns of sample.
Among the various experimental techniques that have been used to investigate DNA binding and flexibility, atomic force microscopy (AFM) has proved to be very efficient.
High-resolution episcopic microscopy (HREM) has proved the most effective of these, using the simple expedient of fluorescent dyes in the plastic embedding medium to obtain very detailed greyscale images from a wide range of biological tissues and optical magnifications [ 25 ].
Stochastic optical reconstruction microscopy (STORM) has proven to be a powerful technique to study both the structure and dynamics of a variety of supramolecular polymers, including BTAs29,35,36.
This configuration is known in X-ray microscopy and has proven to be useful, but has not been applied to neutral atom beams.
To find out more about the mechanisms of bacterial adhesion, atomic force microscopy (AFM) has proven to be the tool of preference in order to determine the forces by which bacteria attach to surfaces and keep themselves adhered (Dufrene 2002; Dorobantu and Gray 2010; Müller and Dufrêne 2011; Dorobantu et al. 2012).
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