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Raman spectroscopy combined with microscopy has a higher resolution (approx. 1 2 µm [26, 30, 42]).
Recent studies [9] have illustrated, however, that microscopy has a very poor sensitivity compared to diagnosis with molecular tools, highlighting that previous studies using these technologies are likely to have significantly underestimated both animal- and herd-level prevalence of these pathogens.
Conventional confocal microscopy has a limited axial resolution of >200 nm.
For this purpose, immunoelectron microscopy has a number of distinct advantages over light-based methods.
Multiphoton microscopy has a number of distinct advantages over the conventional methods for the non-invasive optical detection of AGEs.
The use of SMALPs for structure determination by electron microscopy has a number of distinct advantages over conventional detergent-based techniques.
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SR-DUV fluorescence microscopy has an excitation window down to 190 nm, which allows measuring the natural luminescence of NPs that absorb in the Deep-UV spectral range (below 350 nm).
In HIV-infected patients, sputum smear microscopy has an estimated sensitivity of 35% for active TB [2], and smear-negative TB is associated with worse clinical outcomes than smear-positive disease [3], [4].
We should not forget that microscopy has an unacceptably low sensitivity (2 ).
The sensitivity of urine LAM alone, smear microscopy alone, and urine LAM in combination with smear microscopy (using culture-positivity as a reference standard) is shown in Figure 2. Smear microscopy had a sensitivity of 65%, 49% and 37% in unselected TB patients, in HIV co-infected patients, and in those with a CD4 count <200 cells/mm3 respectively.
Even extended pericyte projections, observed using electron microscopy, have a thin layer of basal lamina.
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